From the posterior part to the anterior part of the AVN, the nucleus dimension and the nucleocytoplasmic ratio of P cells gradually decreased. AVN由后向前,P细胞的核逐渐变小,核浆比下降。
MSCs were isolated from human umbilical cord with the tissue explants adherent method and cultured under normoxic conditions in vitro. The morphological characterization and nucleocytoplasmic ratio of MSCs were observed using Giemsa staining. 2. 方法:1.运用组织块贴壁法从脐带中获得间充质干细胞(MSCs),并在常氧条件下进行传代培养,通过细胞染色观察传代过程中MSCs形态及核质比的变化。